<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div><span>It does not surprise me that the sterilized bags colonize faster. Any bag is like a battleground between the <span id="misspell-1">mycelium</span>, bacteria, and other fungus - a battle for food (i.e. energy) basically. And the less sterile the substrate at spawning, the less microbial advantage conferred to the spawn, although some substrates are only pasteurized because they tend to fill up with bacteria known benign to the species mycelium being grown and also protectively competitive against other bad guys (you know, the enemy of my enemy is inadvertently my friend). </span><span>In terms of energy expenditure, waging a battle is taxing so will slow colonization. In addition heat renders most substrates more imminently digestible to
fungus, thus speeding colonization. I am not sure how this happens chemically or physically, but I have noticed it is particularly true of woody substrates. I also would suspect the Spawn Mate, which is normally a timed release of denatured protein, would "leak" its goodies faster if exposed to a pressure cooker since I do not think it was really designed to be added to a substrate before sterilization or pasteurization, rather only at spawning.</span></div><div><span></span> </div><div><span>Roger</span></div><div><span></span> </div><div><br></div><div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"><div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"><font size="2" face="Arial"><div style="margin: 5px 0px; padding: 0px; border: 1px solid rgb(204, 204, 204); height: 0px; line-height: 0; font-size: 0px;" class="hr" contentEditable="false"
readonly="true"></div><b><span style="font-weight: bold;">From:</span></b> Dana S. <dsniezko@sonic.net><br><b><span style="font-weight: bold;">To:</span></b> tastebridge@lists.noisebridge.net<br><b><span style="font-weight: bold;">Sent:</span></b> Thursday, October 13, 2011 4:16 PM<br><b><span style="font-weight: bold;">Subject:</span></b> Re: [Tastebridge] Coffee grounds for mushrooms<br></font><br>
<div id="yiv1937287517">
<style>#yiv1937287517 BODY {font-family:Arial, Helvetica, sans-serif;font-size:12px;}</style><br>
<br>
<span style="font-weight: bold;"></span><style>#yiv1937287517 BODY {font-family:Arial, Helvetica, sans-serif;}</style>Oh, yes! The bags we cooked have a lot more colonization.<br>
<br>
(Imagine these not blurry or upside-down) <br>
<br>
Pressure cooked: http://i.imgur.com/B4BTL.jpg <br>
<br>
Uncooked: http://i.imgur.com/f2cYK.jpg<br>
<br>
I just realized I'm going to be in Monterey next week, won't be able to make the meeting, so here's my update: I got the low power linux box ( slug, NSLU2) running, we can hook up whatever sensors we want to use (http://linuxaria.com/howto/arduino-serial-ethernet-nslu2?lang=en), a cam with either streamer or webcam (modules already installed, http://nslu2guide.blogspot.com/2010/01/webcam-on-slug.html). It's probably best to upload/store them elsewhere since it's a bit limited -- I am happy to pass along login details to anyone who wants them.<br>
<br>
- Dana <br>
<br>
P.S. a big oyster on one of our old logs: http://i.imgur.com/m6bhu.jpg :)<br>
<br>
<br>
<span style="font-weight: bold;">On Thu 13/10/11 3:13 PM , "Rikke Rasmussen" rikke.c.rasmussen@gmail.com sent:<br>
</span><blockquote style="padding-right: 0px; padding-left: 5px; margin-right: 0px; margin-left: 5px; border-left-color: rgb(245, 245, 245); border-left-width: 2px; border-left-style: solid;"><div>This looks great! Dana, did you see any difference between the bags we pressure cooked and the ones we didn't?</div>
<div>I'm working on a wiki for the project - should be up sometime this weekend.</div>
<div>/Rikke </div>
<div>On Oct 13, 2011 1:51 PM, "Roger H" <<a rel="nofollow">domitron@yahoo.com</a>> wrote:<br></div><blockquote style="margin: 0px 0px 0px 0.8ex; padding-left: 1ex; border-left-color: rgb(204, 204, 204); border-left-width: 1px; border-left-style: solid;" class="yiv1937287517gmail_quote">
<div><div style="color: rgb(0, 0, 0); font-family: times new roman, new york, times, serif; font-size: 12pt; background-color: rgb(255, 255, 255);"><div>That looks like great development. In the picture it seem to be more dense than what I remember seeing in the sawdust-based medium, but that probably is an illusion owing to the relatively high contrast of dark brown coffee grounds to white mycelium. It'll be interesting to see how fruiting compares. Oysters are known to fruit prolifically
on many substrates, so it would not surprise me if this turns out great!</div><div> </div><div>Roger</div><div><br>
</div><div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"><div style="font-family: times new roman, new york, times, serif; font-size: 12pt;">
<span style="font-family: Arial; font-size: 10pt;"><div style="margin: 5px 0px; padding: 0px; border: 1px solid rgb(204, 204, 204); line-height: 0; font-size: 0px; min-height: 0px;"></div><span style="font-weight: bold;">From:</span> Dana S. <<a href="mailto:dsniezko@sonic.net" rel="nofollow" target="_blank" ymailto="mailto:dsniezko@sonic.net">dsniezko@sonic.net</a>><br>
<span style="font-weight: bold;">To:</span> Roger H <<a href="mailto:domitron@yahoo.com" rel="nofollow" target="_blank" ymailto="mailto:domitron@yahoo.com">domitron@yahoo.com</a>>; Dan Willhite <<a href="mailto:willhite@gmail.com" rel="nofollow" target="_blank" ymailto="mailto:willhite@gmail.com">willhite@gmail.com</a>>; Tastebridge <<a href="mailto:tastebridge@lists.noisebridge.net" rel="nofollow" target="_blank" ymailto="mailto:tastebridge@lists.noisebridge.net">tastebridge@lists.noisebridge.net</a>><br>
<span style="font-weight: bold;">Sent:</span> Thursday, October 13, 2011 1:12 PM<br>
<span style="font-weight: bold;">Subject:</span> Re: [Tastebridge] Coffee grounds for mushrooms<br>
</span><br>
<div>
It's getting there!<br>
<br>
Taken a few moments ago: http://i.imgur.com/zWzmI.jpg<br>
<br>
- Dana<br>
<br>
<br>
<span style="font-weight: bold;">On Thu 13/10/11 10:51 AM , "Roger H" <a href="mailto:domitron@yahoo.com" rel="nofollow" target="_blank" ymailto="mailto:domitron@yahoo.com">domitron@yahoo.com</a> sent:<br>
</span><blockquote style="padding-right: 0px; padding-left: 5px; margin-right: 0px; margin-left: 5px; border-left-color: rgb(245, 245, 245); border-left-width: 2px; border-left-style: solid;"><div style="color: rgb(0, 0, 0); font-family: times new roman, new york, times, serif; font-size: 12pt; background-color: rgb(255, 255, 255);">
<div>Has anyone noted the level of colonization in the coffee grind bags? I am curious if they are coming along as well as the sawdust bags did, which were basically 100% colonized in about five days.</div><div> </div><div>
Roger</div><div> </div><div><br>
</div><div style="font-family: times new roman, new york, times, serif; font-size: 12pt;">
<div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"><span style="font-family: Arial; font-size: 10pt;"><div style="margin: 5px 0px; padding: 0px; border: 1px solid rgb(204, 204, 204); line-height: 0; font-size: 0px; min-height: 0px;">
</div><span style="font-weight: bold;">From:</span> Roger H <br>
<span style="font-weight: bold;">To:</span> Dan Willhite ; Tastebridge <br>
<span style="font-weight: bold;">Sent:</span> Monday, October 10, 2011 12:36 PM<br>
<span style="font-weight: bold;">Subject:</span> Re: [Tastebridge] Coffee grounds for mushrooms<br>
</span><br>
<div><div><div style="color: rgb(0, 0, 0); font-family: times new roman, new york, times, serif; font-size: 12pt; background-color: rgb(255, 255, 255);"><div>If we go with wet grounds, we must adjust the moisture with vermiculite, which I have several cubic feet. Even if we must use 50% vermiculite per volume, I think we'll be fine if we supplement with the Spawn Mate II, which I will bring. I will also bring bags, hydrogen peroxide, digital scales, a syringe, oyster spawn, and sealer.<br>
</div><div><br>
</div><div>Roger</div><div><br>
</div><div><br>
</div><div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"><div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"><span style="font-family: Arial; font-size: 10pt;"><div style="margin: 5px 0px; padding: 0px; border: 1px solid rgb(204, 204, 204); line-height: 0; font-size: 0px; min-height: 0px;">
</div><span style="font-weight: bold;">From:</span> Dan Willhite <br>
<span style="font-weight: bold;">To:</span> Tastebridge
<br>
<span style="font-weight: bold;">Sent:</span> Monday, October 10, 2011 11:00 AM<br>
<span style="font-weight: bold;">Subject:</span> [Tastebridge] Coffee grounds for mushrooms<br>
</span><br>
<div><br>
<div>Hi mushroom growers,</div><div><br>
</div><div>I committed to delivering 10-20 pounds of dried, used coffee grounds tonight. Unfortunately, the weather was unsuitable for drying the grounds this week on the days that I would have been able to dry them. The weather outlook looks good for this week but unfortunately I won't be able to have them for tonight. I could still try to obtain some grounds today but I won't be able to dry them. I'm not sure if it makes sense for me to bring more wet grounds tonight since someone else has already committed to bring them but I'm happy to try to obtain them if someone thinks they'd be useful. </div>
<div><br>
</div><div>--Danny</div>
</div><br>
_______________________________________________<br>
Tastebridge mailing list<br>
<a rel="nofollow">Tastebridge@lists.noisebridge.net</a><br>
<a href="https://www.noisebridge.net/mailman/listinfo/tastebridge" rel="nofollow" target="_blank">https://www.noisebridge.net/mailman/listinfo/tastebridge</a><br>
<br>
<br>
</div></div></div></div></div><br>
_______________________________________________<br>
Tastebridge mailing list<br>
<a rel="nofollow">Tastebridge@lists.noisebridge.net</a><br>
<a href="https://www.noisebridge.net/mailman/listinfo/tastebridge" rel="nofollow" target="_blank">https://www.noisebridge.net/mailman/listinfo/tastebridge</a><br>
<br>
<br>
</div></div></div>
_______________________________________________<br>
Tastebridge mailing list<br>
<a rel="nofollow">Tastebridge@lists.noisebridge.net</a><br>
<a rel="nofollow"><span style="color: red;">https://www.noisebridge.net/mailman/listinfo/tastebridge</span></a><br>
<br>
<br>
</blockquote>
</div><br>
<br>
</div></div></div></div><br>
_______________________________________________<br>
Tastebridge mailing list<br>
<a rel="nofollow">Tastebridge@lists.noisebridge.net</a><br>
<a href="https://www.noisebridge.net/mailman/listinfo/tastebridge" rel="nofollow" target="_blank">https://www.noisebridge.net/mailman/listinfo/tastebridge</a><br>
<br>
</blockquote>
</blockquote>
</div><br>_______________________________________________<br>Tastebridge mailing list<br><a href="mailto:Tastebridge@lists.noisebridge.net" ymailto="mailto:Tastebridge@lists.noisebridge.net">Tastebridge@lists.noisebridge.net</a><br><a href="https://www.noisebridge.net/mailman/listinfo/tastebridge" target="_blank">https://www.noisebridge.net/mailman/listinfo/tastebridge</a><br><br><br></div></div></div></body></html>