[Noisebridge-discuss] Shaker/Incubator: May I get some help with mechanical construction

[Glen Jarvis]

You're videos are awesome! They help me communicate what I want to do. It
also helps me see there are more kinds of shakers than the kind that I have
watched other people use (and it shows me the names of them - I didn't know
I was talking about an orbital shaker).

Also, you're right, I can move the sample to the smaller size - the mason
jar example was too large. I was thinking of cooking up a bottle of LB and
what it would fit in while in an incubator/shaker. However, I'm dealing with
such smaller volumes, I don't need to think of that level...

Unfortunately, the sales prices that I saw were in the $300+ range. I'm
hoping to make something very similar for < $50...  I'd rather buy so I can
actually get on with the experiment I'm trying to do... Alas, I can't afford
that luxury :(

I have a much smaller budget than those large grant underwritten
institutions :(


Glen

On Wed, Apr 28, 2010 at 10:06 PM, Sean Cusack <sean.p.cusack at gmail.com>wrote:

> Nice - those both sound like awesome ideas! Man I wish that they taught me
> how to do cheap skate lab stuff in skool :).
>
> Sean
>
>
> On Wed, Apr 28, 2010 at 10:02 PM, Glen Jarvis <glen at glenjarvis.com> wrote:
>
>> This is awesome -- I'm still absorbing it all, but let me answer a quick
>> question:
>>
>>  One random question - have you thought about sterility?? If you're going
>>> to try and grow some bugs at home you will undoubtedly contaminate pretty
>>> much every other crop you make with some random bacteria and totally hose
>>> your experiment. You probably want to consider hacking together some sort of
>>> "bio area" that has a germicidal lamp in it so you can nuke those little
>>> bacterial bastards: http://www.topbulb.com/find/germicidal.asp
>>
>>
>>
>> Oh, this side is down. I use a pressure cooke as an autoclave and have had
>> great results. I cook up some medium, sterilize glass petri dishes... and am
>> ready to go. I have some ecoli now in a colony count :)
>>
>> The pressure cooker is definitely the way to go unless you can buy an
>> autoclave:
>>
>> http://www.science-projects.com/PressureCooker.htm
>>
>>
>> Also, although LB Medium is the standard for e.coli, MacConkey  (
>> http://en.wikipedia.org/wiki/MacConkey_agar) is very forgiving for
>> an environment at home (http://www.science-projects.com/MacConkeyNS.htm).
>>
>>
>> <http://www.science-projects.com/PressureCooker.htm>Cheers,
>>
>>
>> Glen
>>
>>
>>
>> --
>> Whatever you can do or imagine, begin it;
>> boldness has beauty, magic, and power in it.
>>
>> -- Goethe
>>
>
>


-- 
Whatever you can do or imagine, begin it;
boldness has beauty, magic, and power in it.

-- Goethe
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