[Noisebridge-discuss] Shaker/Incubator: May I get some help with mechanical construction

[Glen Jarvis]

This is awesome -- I'm still absorbing it all, but let me answer a quick
question:

One random question - have you thought about sterility?? If you're going to
> try and grow some bugs at home you will undoubtedly contaminate pretty much
> every other crop you make with some random bacteria and totally hose your
> experiment. You probably want to consider hacking together some sort of "bio
> area" that has a germicidal lamp in it so you can nuke those little
> bacterial bastards: http://www.topbulb.com/find/germicidal.asp



Oh, this side is down. I use a pressure cooke as an autoclave and have had
great results. I cook up some medium, sterilize glass petri dishes... and am
ready to go. I have some ecoli now in a colony count :)

The pressure cooker is definitely the way to go unless you can buy an
autoclave:

http://www.science-projects.com/PressureCooker.htm


Also, although LB Medium is the standard for e.coli, MacConkey  (
http://en.wikipedia.org/wiki/MacConkey_agar) is very forgiving for
an environment at home (http://www.science-projects.com/MacConkeyNS.htm).


<http://www.science-projects.com/PressureCooker.htm>Cheers,


Glen



-- 
Whatever you can do or imagine, begin it;
boldness has beauty, magic, and power in it.

-- Goethe
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